NCI-H446 In Vitro High-throughput Screening (HTS) Assay …
This assay is to provide NCI-H446-based In Vitro High-throughput Screening (HTS) Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Semi-Automated High-Throughput Substrate Screening Assay …
The developed high-throughput assay revealed comparable results to HPLC analysis. The assay was exemplarily used for the comparison of the substrate spectra of four NKs using 20 (8 natural, 12 modified) substrates.
(PDF) Integrated Model of Chemical Perturbations of a …
Integrated Model of Chemical Perturbations of a Biological Pathway Using 18 In Vitro High Throughput Screening Assays for the Estrogen Receptor. August 2015; Toxicological Sciences 148(1)
Risk-Based High-Throughput Chemical Screening and …
chemicals.3 For this reason, high-throughput screening (HTS) assessments that incorporate both exposure and toxicity data are recommended for risk-based screening and prioritization.4 The U.S. Environmental Protection Agency (EPA) has developed a process combining in vitro HTS assays with computational tools to …
High-Throughput Screening to Advance In Vitro Toxicology
The Tox21 program, a unique research consortium of federal public health agencies, was established to address and identify toxicity concerns in a high …
Design considerations for high-throughput screening and in vitro …
This paper reviews the several different factors that must be considered during the development of assays for high throughput screening (HTS) or in vitro diagnostic (IVD) applications. The reader is introduced to the terminology used in assay development as well as the statistical approaches for eva …
High-Throughput Screening to Predict Chemical-Assay …
The U.S. federal consortium on toxicology in the 21st century (Tox21) produces quantitative, high-throughput screening (HTS) data on thousands of chemicals across a wide range of assays covering ...
A High-Throughput In Vitro Assay for Screening Rice …
A High-Throughput In Vitro Assay for Screening Rice Starch Digestibility Foods. 2019 Nov 21 ... The objective of this study was to develop a high-throughput in vitro assay to screen the digestibility of cooked white rice grains and to investigate its ability to differentiate rice genotypes with a low starch digestibility rate. …
High Throughput Screening
High Throughput Screening. M.S. Attene-Ramos, ... M. Xia, in Encyclopedia of Toxicology (Third Edition), 2014 Definition. High throughput screening (HTS) is the use of automated equipment to rapidly test thousands to millions of samples for biological activity at the model organism, cellular, pathway, or molecular level. In its most common form, HTS is an …
In vitro high-throughput screening assay for modulators of
We developed a 96-well microtiter-plate high-throughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur …
High-Throughput Screening (HTS)
HTS is the process of screening large numbers of biological modulators against selected targets. HTS systems are used to screen different types of libraries such as chemical, …
High-throughput screening in colorectal cancer tissue …
Patient-derived cancer organoid culture is an important live material that reflects clinical heterogeneity. However, the limited amount of organoids available for each case as well as the considerable amount of time and cost to expand in vitro makes it impractical to perform high-throughput drug screening using organoid cultures from multiple patients.
Transforming early pharmaceutical assessment of …
To provide a comprehensive analysis of small molecule genotoxic potential we have developed and validated an automated, high-content, high throughput, image-based in vitro Micronucleus (IVM) assay. This assay simultaneously assesses micronuclei and multiple additional cellular markers associated wit …
High-Throughput Screening (HTS)
Biomek workstations provide comprehensive, tailored solutions for high-throughput screening workflows. Beyond robust instrumentation, we provide data-rich software, deep workflow and application expertise, custom engineering, service plans and a broad range of supporting resources. Our systems can meet virtually all high-throughput screening …
High-Throughput Screening Assays and In Vitro Bioassays
Science method High-Throughput Screening Assays. Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way ...
HT29 In Vitro High-throughput Screening (HTS) Assay
This assay is to provide HT29-based In Vitro High-throughput Screening (HTS) Assay to accelerate our client's oncology projects. The assay will be customized according to the …
High-throughput Screening (HTS) Assay | Charles …
High-throughput mass spectrometry (HTMS) can be applied in screening and is a process whereby eliminating artifacts introduced by fluorescent tags, label-free biochemical and cell-based screens generate rich, multi …
High Throughput Screening
G-protein-coupled receptor screening technologies. Peter J. Greasley, Frank P. Jansen, in Drug Discovery Today: Technologies, 2005 High throughput screening (HTS) plays a crucial role in lead-generation and is well established in the preclinical discovery process of pharmaceutical companies [1,2].Although primary screening is more than just a 'large …
Computational models for in-vitro anti-tubercular activity of …
In the present study, we have used the data from in-vitro screens for anti-tubercular activity from a high-throughput screen available in public domain to build highly accurate classifiers based on molecular descriptors of the molecules. We show that Machine Learning tools can be used to build highl …
MB49 In Vitro High-throughput Screening (HTS) Assay
This assay is to provide MB49-based In Vitro High-throughput Screening (HTS) Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. The assay will be customized according to the specific requirements.
HTS Assay Validation
This chapter addresses the essential statistical concepts and tools needed in assay performance validation developed in the pharmaceutical industry, specifically for …
Foods | Free Full-Text | A High-Throughput In Vitro Assay for Screening …
The development of rice that can produce slow and steady postprandial glucose in the bloodstream is a response to alarmingly high global rates of obesity and related chronic diseases. However, rice grain quality programs from all over the world currently do not have access to a high-throughput method to distinguish rice breeding …
High-Throughput Screening
NUVISAN's experienced scientists design and execute cost-effective, miniaturized assays for high-throughput screening, leveraging state-of-the-art equipment and a vast …
C1498 In Vitro High-throughput Screening (HTS) Assay
This assay is to provide C1498-based In Vitro High-throughput Screening (HTS) Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Closing the empty anti-Babesia gibsoni drug pipeline in vitro …
In the current study, we evaluated the usefulness of a SYBR Green I (SG I) fluorescence assay for evaluation of the inhibitory effects of antibabesial drugs against the in vitro growth of Babesia gibsoni. Linearity and high-throughput screening (HTS) assays exhibited the validity of the SG I fluores …
High-Throughput Screening Technology in Industrial …
We discuss the most significant factors that can improve HTS efficiency, including the construction of screening libraries with high diversity and the use of new detection …
High-throughput screening for analysis of in vitro toxicity
The influence of combinatorial chemistry and high-throughput screening (HTS) technologies in the pharmaceutical industry during the last 10 years has been enormous. However, the attrition rate of drugs in the clinic due to …
AGM eBook | National Center for Advancing Translational …
The Assay Guidance Manual (AGM) eBook is a free, best-practices online resource devoted to the successful development of robust, early-stage drug discovery assays.. The manual was adapted from documents developed by the drug discovery industry (Eli Lilly and Company and Sphinx Pharmaceuticals, Inc.) that were designed …
A High-Throughput In Vitro Assay for Screening Rice …
3.1. A High-Throughput In Vitro Assay Proposed Specifically for the Digestibility of Cooked Rice. To allow for rapid screening of samples, modifications were made to the in vitro digestion assay (as described in Section 2.4). Sampling was only taken at a single time point (60 min), and digesta aliquots were immediately heated to a …
High-Throughput Screening (HTS) Technology | SpringerLink
Definition. HTS is a translational research technology used for identifying effectors (compounds, peptides, and biologics) that modulate target-specific biochemical …