Adhesion of Pseudomonas fluorescens (ATCC 17552) to
The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two different ionic strengths (0.01 and 0.1 M NaCl; pH 7) under controlled …
Adhesion of Pseudomonas fluorescens (ATCC 17552) to …
The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy …
Nanoscale Adhesion Forces of Pseudomonas aeruginosa …
A variety of bacterial pathogens use nanoscale protein fibers called type IV pili to mediate cell adhesion, a primary step leading to infection. Currently, how these nanofibers respond to mechanical stimuli and how this response is used to control adhesion is poorly understood. Here, we use atomic force microscopy techniques to …
The effect of burdock leaf fraction on adhesion, biofilm …
This study aimed to evaluate the effect of a fraction of burdock (Arctium lappa L.) leaf on the initial adhesion, biofilm formation, quorum sensing and virulence factors of Pseudomonas aeruginosa. Methods and Results. Antibiofilm activity of the burdock leaf fraction was studied by the method of crystal violet staining.
Genetic analysis of functions involved in adhesion of Pseudomonas
Pseudomonas putida KT2440 is a strain that colonizes the rhizosphere of a number of agronomically importa … Genetic analysis of functions involved in adhesion of Pseudomonas putida to seeds J Bacteriol. 2000 May;182(9):2363-9. doi: 10.1128/JB.182.9.2363-2369.2000. ...
Development of a high throughput Pseudomonas aeruginosa …
1.. IntroductionPseudomonas aeruginosa (PA) causes severe pulmonary infections in immunocompromised patients and in patients with cystic fibrosis (CF) Hoiby, 1993, Hutchison and Govan, 1999. P. aeruginosa is the major culprit responsible for the morbidity and mortality seen in CF patients (Baltimore et al., 1989).Adhesion of PA to …
Adhesion of Pseudomonas putida onto kaolinite at …
This work examined the influence of growth phase on the adhesion of Pseudomonas putida to kaolinite-coated coverslips. Fluorescence microscopy, together with a bacterial viability stain, was used to directly quantify surface cell density and viability of adhered P. putida. In situ attenuated total reflectance Fourier transform infrared ...
Nanoscale adhesion forces of Pseudomonas aeruginosa type …
Here, we use atomic force microscopy techniques to quantify the forces guiding the adhesion of Pseudomonas aeruginosa type IV pili to surfaces. Using chemical force microscopy and single-cell force spectroscopy, we show that pili strongly bind to hydrophobic surfaces in a time-dependent manner, while they weakly bind to hydrophilic …
Pseudomonas fluorescens Migula
To download a certificate of origin for Pseudomonas fluorescens Migula (13459), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Origin. Certificate of Origin Request The certificate of ...
Fitness in soil and rhizosphere of Pseudomonas fluorescens …
Transposon mutant strain 3G6 of Pseudomonas fluorescensATCC 17400, which was deficient in pyoverdine production, was found to produce another iron-chelating molecule, identified as 8-hydroxy-4-methoxy-quinaldic acid (designated quinolobactin), which is assumed to be the 75-kDa iron-repressed outer membrane protein (IROMP).
Development of a high throughput Pseudomonas …
Pseudomonas aeruginosa colonizes the lungs of cystic fibrosis and mechanically ventilated patients by binding to specific carbohydrate residues on the surface of lung epithelial cells. Studies have shown that blocking this interaction may have therapeutic effects in vivo. ... we have developed a pseudomonal adhesion assay that is compatible ...
Growth temperature and OprF porin affect cell surface
Pseudomonads adapt to various ecological niches by forming biofilms, which first requires bacterial adhesion on surfaces. We studied the influence of growth temperature on surface physicochemical properties of Pseudomonas fluorescens MF37 and on its adhesive capacities onto inert surfaces. It presented a global hydrophilic …
Hypertonic stress induced changes of Pseudomonas fluorescens adhesion
Using atomic force microscopy (AFM), we investigated the influence of hypertonic stress on Pseudomonas fluorescens adhesion to four different minerals in water. These findings were compared with ...
Adhesion of Pseudomonas fluorescens (ATCC 17552) to …
The goals of this review are: (1) to present the current status of knowledge regarding electrochemical biofilm control; (2) to establish a basis for a fundamental definition of …
Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit …
Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2′-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 ...
Adhesion of Pseudomonas fluorescens on magnetic surfaces
The adhesion of Pseudomonas fluorescens (ATCC 700830) to perpendicularly polarized magnetic surfaces was recently discovered. The findings have …
Shear force enhances adhesion of Pseudomonas …
Bacteria experience fluid flow in natural environments and during host colonization. As a result, many bacterial species have evolved elaborate mechanisms to colonize environments with high shear forces (1 –6).For example, Escherichia coli uses the protein FimH to promote adhesion in the human urinary tract (7 –9).FimH is remarkable …
Adhesion to hyphal matrix and antifungal activity of Pseudomonas
Pseudomonas spp. isolates from Tuber borchii ascocarps, known to be able to produce phytoregulatory and biocontrol substances in pure culture, were used to perform studies on their possible physiological role in nature. ... Adhesion to hyphal matrix and antifungal activity of Pseudomonas strains isolated from Tuber borchii ascocarps Can J ...
Adhesion of Pseudomonas aeruginosa and
In vitro static adhesion assays of Pseudomonas aeruginosa 10,145, Staphylococcus epidermidis 9142 (biofilm-positive), and 12,228 (biofilm-negative) to two extended-wear silicone–hydrogel lenses (balafilcon A and lotrafilcon A), a daily wear silicone–hydrogel lens (galyfilcon A) and a conventional hydrogel (etafilcon A) were …
Genetic Analysis of Functions Involved in Adhesion of Pseudomonas
Soil bacteria belonging to the species Pseudomonas fluorescens and Pseudomonas putida show metabolic versatility and a variety of characteristics that makes them attractive for environmental and agricultural uses ().They can colonize the surface of plant roots and the surrounding soil regions (rhizosphere) in a mutualistic association in …
Factors influencing adhesion of Pseudomonas putida on …
The aim of the study was to determine factors affecting adhesion of bacteria Pseudomonas putida on porous clay ceramic granules. The number of colony-forming units (CFU) of P. putida on Devonian clay granules sintered at temperature of 1100 °C after 4-h-long incubation was estimated. The level of the adhesion significantly (P < 0.05) …
Electrochemical Polarization-Induced Changes in the Growth …
The aims of the present work were to determine both the effects of low-amplitude direct-current polarization on the growth and division of individual bacterial cells of …
Identification of reciprocal adhesion genes in pathogenic …
We used a combination of in silico and large-scale mutagenesis approaches to expand our current knowledge of the genetic determinants used by Pseudomonas putida KT2440 to attach to surfaces. We first identified in silico orthologues that have been annotated in Pseudomonas aeruginosa as potentially involved in attachment. In this …
The Application of Bacillus subtilis for Adhesion Inhibition of
Inhibiting the growth of spoilage bacteria, such as Pseudomonas spp., is key to reducing spoilage in fish. The mucus adhesion test in vitro showed that the adhesion ability of Bacillus subtilis was positively correlated with its inhibition ability to Pseudomonas spp. In vivo experiments of tilapia showed that dietary supplementation …
Shear force enhances adhesion of Pseudomonas …
Bacteria experience fluid flow in natural environments and during host colonization. As a result, many bacterial species have evolved elaborate mechanisms to colonize environments with high shear forces (1–6).For example, Escherichia coli uses the protein FimH to promote adhesion in the human urinary tract (7–9).FimH is remarkable as it …
The Epidemiology and Pathogenesis and Treatment of Pseudomonas
Introduction. Pseudomonas aeruginosa is a Gram-negative pathogen that is commonly associated with hospital-acquired infections, infections in immunocompromised hosts, and chronic infections in patients with structural lung disease such as cystic fibrosis (CF). For this review, we sought to update the readers with regard to the epidemiology …
Prevention of Pseudomonas aeruginosa adhesion by …
The process of controlling bacterial adhesion using an electric current deserves attention because of its ease of automation and environmentally friendly nature. This study investigated the role of electric currents (negative, positive, alternating) for preventing adhesion of Pseudomonas aeruginosa …
The influence of the surface condition on the adhesion of …
The adhesion of P. fluorescens (ATCC 17552) to copper, zinc and aluminium brass in the absence and presence of a surface oxide layer was evaluated performing …
The Application of Bacillus subtilis for Adhesion Inhibition …
Inhibiting the growth of spoilage bacteria, such as Pseudomonas spp., is key to reducing spoilage in fish. The mucus adhesion test in vitro showed that the adhesion ability of Bacillus subtilis was positively correlated with its inhibition ability to Pseudomonas spp. In vivo experiments of tilapia showed that dietary supplementation …
Impact of the exopolysaccharides Pel and Psl on the initial adhesion …
Abstract. In this study, the impact of the exopolysaccharides Pel and Psl on the cell surface electron donor-electron acceptor (acid-base) properties and adhesion to quartz sand was investigated by using Pseudomonas aeruginosa PAO1 and its isogenic EPS-mutant strains Δpel, Δpsl and Δpel/Δpsl.The microbial adhesion to hydrocarbon …