Viruses | Free Full-Text | Viral Aggregation: The …
Viral aggregation is a complex and pervasive phenomenon affecting many viral families. An increasing number of studies have indicated that it can modulate critical parameters surrounding viral …
Flexible resource dynamic aggregation regulation method …
In Comparison to the first method, the second method achieved a cost reduction of 18.19 × 102$. and the NICCDE method calculated a better cost, in order to facilitate calculation, this paper weakens the effect of interruptible load in VPP on the output of a carbon capture power plant, that is, the equivalent output of the carbon capture …
Advances in protein-based diagnostic tools of plant viruses
Microscopic methods to detect plant viruses using electron microscopy or light microscopy is simple and rapid, ... Detection of hepatitis B surface antigen by target-induced aggregation monitored by dynamic light …
Progressive aggregation of Tomato yellow leaf curl virus …
Viral replication, transcription, translation and movement, as well as virus inoculum dose and inoculation methods, influence the aggregation level of TYLCV CP in tomato plants [31]. Different ...
Viruses | Free Full-Text | Viral Aggregation: The Knowns and …
Viral aggregation is a complex and pervasive phenomenon affecting many viral families. An increasing number of studies have indicated that it can modulate critical parameters surrounding viral infections, and yet its role in viral infectivity, pathogenesis, and evolution is just beginning to be appreciated. Aggregation likely promotes viral infection …
A novel colorimetric biosensor for rapid detection of dengue virus …
Dengue virus serotype 1 was utilized as a framework for virus detection; the designed nanosensor exhibited great specificity and selectivity, with the detection limit of ∼1 pg μL −1 (∼1.66 × 10 6 RNA copies per reaction) and time of analysis of about 1 h including the RNA extraction step. The proposed colorimetric nanosensor offers an ...
Virus reconstitution and the proof of the existence of …
began to characterize a virus by such methods, he believed it to be a protein (Stanley 1935). Only a few ... amount of RNA in some plant viruses such as TMV (5%) could have any biological signi¢cance. ... repeated Schramm's native-protein aggregation experi-ments, but added to the reaction mixture the intact RNA obtained by detergent (SDS ...
Transmission of plant viruses in fields through various vectors
The majority of the viruses causing disease in plants are transmitted from infected to healthy plants through insect vectors. Although the relationship between plant viruses and their insect ...
A general model of plant‐virus disease infection
In plant-virus disease epidemiology, dynamical models have invariably incorporated a bilinear inoculation rate that is directly proportional to both the abundance …
Exploitation of plant and archaeal viruses in …
CPMV (cowpea mosaic virus), a plant virus, is a naturally occurring sphere-like nanoparticle, and is used as a synthon and/or template in bionanoscience. ... and a procedure has been developed that enables introduction of reactive thiols at the virion surface that avoids virus aggregation. Furthermore, the virions can be functionalized to …
X-RAY AND CRYSTALLOGRAPHIC STUDIES OF PLANT VIRUS …
x-ray and crystallographic studies of plant virus preparations : i. introduction and preparation of specimens ii. modes of aggregation of the virus particles
Purification of potato virus X without aggregation
Virus PURIFICATION OF POTATO VIRUS X 15 multiplication in plants doubly infected by potato viruses X and Y. Virology 1,10-27. ROCHOW, W. F., Ross, A. F., and SIEGEL, B. M. (1955). Comparison of local-lesioa and electron-microscope particle-count methods for assay of potato virus X from plants doubly infected by potato viruses X and Y. Virology ...
Virus-like Particles: Fundamentals and Biomedical Applications
3. Key Concepts about Virus-like Particles (VLPs) The assembly of VLPs uses exclusively viral proteins and excludes the genetic material. Thus, VLPs can be produced from a myriad of wild-type viruses, such as hepatitis B and E, tobacco, and papaya mosaic viruses [], and from the capsids of various bacteriophages (Qβ, MS2, …
Transmission of Plant Viruses: 8 Methods | Virology
ADVERTISEMENTS: The following points highlight the eight chief methods used for the transmission of plant viruses. The methods are: 1. Seed Transmission of Virus 2. Transmission by Vegetative Propagation 3. Transmission by Mechanical Means 4. Transmission by Cuscuta 5. Soil Transmission 6. Insect Transmission 7. Transmission …
Plant Virus Serology
The accelerated development of serological studies on plant viruses during the past ten years can be followed easily by simply counting the number of viruses that have proved amenable to serological testing. This steady increase partly reflects the widening applicability and improvements of virus purification methods and seems to allow the ...
Environmental Stresses Induce Misfolded Protein Aggregation in Plant
Thus, in the case of plant virus infection, microtubules may play a role in the aggregation of proteins in the cytoplasm of plant cells. In this study, we examined protein aggregation in plant cells exposed to environmental stresses. Environmental stresses—including UV radiation, heat stress, and cold stress—induced ER stress in …
Virus-Induced Aggregates in Infected Cells
Virus-induced membrane structures, mostly shown for plant RNA viruses, house the RNA replication complex and may be compared with virus factories in infected mammalian cell [3,9], even though plant cells lack MTOC, the most characteristic component of virus factories. Interestingly, these structures were identified as a …
Disease outbreaks in plant-vector-virus models with vector aggregation …
While feeding on host plants, viruliferous insects serve as vectors for viruses. Successful viral transmission depends on vector behavior. Two behaviors that impact viral transmission are vector aggregation and dispersal. Vector aggregation may be due to chemical or visual cues or feeding preferences. Vector dispersal can result in widespread disease …
Methods for long-term virus preservation
The methods by which viruses may be preserved for long periods of time are similar to those employed for other microorganisms and are relatively simple. Nevertheless, attention to detail, good laboratory practice, aseptic technique, meticulous recordkeeping, and regular monitoring of the stored materials will increase the success rate and ...
Purification of plant viruses and virus coat proteins by high
In conclusion, HPLC gel filtration of either intact or dissociated plant viruses can be used as a fast and simple last step in a virus purification scheme to provide very pure material for immunization or other purposes. In particular for labile or aggregation-prone viruses this would seem to be the method of choice.
Effect of protein aggregation state on coat protein …
Semantic Scholar extracted view of "Effect of protein aggregation state on coat protein-mediated protection against tobacco mosaic virus using a transient protoplast assay." ... The most promising method for genetic engineering of plants for virus resistance has been the expression of coat-protein coding sequences in plants transformed with a ...
A general model of plant-virus disease infection …
ecology and evolution of plant viruses is particularly complex because most are transmitted between plants by vectors. A three-way interaction exists between virus, host plant and vector, and transmission of viruses is controlled by two processes: acquisition of virus from infected plants; and inoculation of healthy host plants by vectors.
Whitefly aggregation on tomato is mediated by feeding
Bemisia tabaci exhibits a preference for plants attacked by conspecifics.B. tabaci foraging (a) and oviposition (b) preferences after different durations of B. tabaci infestation.B. tabaci foraging (c) and oviposition (d) preferences for wounded plants application of artificial diet with and without B. tabaci saliva.B. tabaci foraging (e) and …
Viral Aggregation: Impact on Virus Behavior in the Environment
Aggregates of viruses can have a significant impact on quantification and behavior of viruses in the environment. Viral aggregates may be formed in numerous ways. Viruses may form crystal like structures and aggregates in the host cell during replication or may form due to changes in environmental conditions after virus particles are released from …
Viral Aggregation: The Knowns and Unknowns
More than eight decades ago, the first studies reported aggregates of plant-infecting tobacco mosaic virus (TMV, family Virgaviridae) ... preventing charge shielding and virus aggregation [34,39]. Similarly, temperature influences viral aggregation, with more aggregates forming at higher temperatures. Viruses are colloidal particles, and as ...
Epidemiological and ecological consequences of virus …
Introduction. Plant diseases have impacts on crops, affecting yield, and on natural plant ecosystems and landscapes, affecting population and community structure [1–3].Plant diseases caused by viruses can be particularly damaging [].Most plant viruses are transmitted by vectors, which both acquire virus from infected plants and inoculate …
Viruses in wastewater: occurrence, abundance and detection methods
Recent efforts to improve efficiency of virus detection and quantification methods in the complex wastewater and sludge matrices are highlighted in this review. A summary and a detailed comparison of the pre-treatment methods that have been utilized for wastewater and sludge samples are also presented. The role of metagenomics or …
4 Isolation and Purification of Plant Viruses
42 PLANT VIRUSES material, but for consistently successful purification a larger quantity than this is desirable. Another important point is the plant used as a source of virus; many factors are concerned here. As a rule, young plants, showing recent systemic symptoms, have a higher virus content than old plants long infected, and contain
Vector acquisition and co-inoculation of two plant viruses
Co-infections of plant viruses can be propagated by vectors that acquired virions from co-infected plants, sequentially from singly infected plants, or by multiple …
Application of nanoparticles for management of plant viral …
Patents related to nanomaterial used for plant virus control. The method of inhibiting a plant virus using gold nanoparticles has been patented (US9198434B1) by King Saud University, Riyadh (SA), by inventors- Noorah Abdulaziz Othman Alkubaisi, Nagwa Mohamed Mohamed Amin Aref, and Awatif Ahmed Hendi (2014), The work has …